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Expression of <t>CHAF1A</t> and its correlation with neonatal body composition. A Immunohistochemistry of CHAF1A expression; B Analysis of immunohistochemical results; C Western blotting of CHAF1A expression; D Pearson’s coefficient correlation analysis. NN Normal glucose with normal weight group; GM Gestational diabetes mellitus with macrosomia; F% body fat percentage; SFT4 the sum of bicipital, tricipital, subscapular, and suprailiacal skinfold thickness
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Expression of CHAF1A and its correlation with neonatal body composition. A Immunohistochemistry of CHAF1A expression; B Analysis of immunohistochemical results; C Western blotting of CHAF1A expression; D Pearson’s coefficient correlation analysis. NN Normal glucose with normal weight group; GM Gestational diabetes mellitus with macrosomia; F% body fat percentage; SFT4 the sum of bicipital, tricipital, subscapular, and suprailiacal skinfold thickness

Journal: Reproductive Sciences

Article Title: CHAF1A Promotes Preadipocyte Differentiation and Contributes to Macrosomia in Gestational Diabetes Mellitus

doi: 10.1007/s43032-025-01946-z

Figure Lengend Snippet: Expression of CHAF1A and its correlation with neonatal body composition. A Immunohistochemistry of CHAF1A expression; B Analysis of immunohistochemical results; C Western blotting of CHAF1A expression; D Pearson’s coefficient correlation analysis. NN Normal glucose with normal weight group; GM Gestational diabetes mellitus with macrosomia; F% body fat percentage; SFT4 the sum of bicipital, tricipital, subscapular, and suprailiacal skinfold thickness

Article Snippet: The primary antibodies included: CHAF1A (1:1000), PPAR-γ (16,643–1-AP, 1:1000, Proteintech), SREBP1 (14,088–1-AP, 1:1000, Proteintech), FASN (10,624–2-AP, 1:1000, Proteintech), CEBPα(29,388–1-AP, 1:1000, Proteintech), LPL (28,602–1-AP, 1:1000, Proteintech) and β-Actin (66,009–1-Ig, 1:1000, Proteintech) as a loading control.

Techniques: Expressing, Immunohistochemistry, Immunohistochemical staining, Western Blot

Effects of CHAF1A overexpression on preadipocyte proliferation and differentiation. A Cell morphology under fluorescence microscope; B CHAF1A mRNA; C Western blotting; D CCK8 assays; E Glycerin triglyceride content assays; F Oil Red O staining. *** P < 0.001, ** P < 0.01, * P < 0.05

Journal: Reproductive Sciences

Article Title: CHAF1A Promotes Preadipocyte Differentiation and Contributes to Macrosomia in Gestational Diabetes Mellitus

doi: 10.1007/s43032-025-01946-z

Figure Lengend Snippet: Effects of CHAF1A overexpression on preadipocyte proliferation and differentiation. A Cell morphology under fluorescence microscope; B CHAF1A mRNA; C Western blotting; D CCK8 assays; E Glycerin triglyceride content assays; F Oil Red O staining. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The primary antibodies included: CHAF1A (1:1000), PPAR-γ (16,643–1-AP, 1:1000, Proteintech), SREBP1 (14,088–1-AP, 1:1000, Proteintech), FASN (10,624–2-AP, 1:1000, Proteintech), CEBPα(29,388–1-AP, 1:1000, Proteintech), LPL (28,602–1-AP, 1:1000, Proteintech) and β-Actin (66,009–1-Ig, 1:1000, Proteintech) as a loading control.

Techniques: Over Expression, Fluorescence, Microscopy, Western Blot, Staining

Effects of CHAF1A overexpression on key factors involved in preadipocyte differentiation. A PPAR-γ, SREBP1, FASN, CEBPα, and LPL mRNA; B Western blotting. *** P < 0.001, ** P < 0.01, * P < 0.05

Journal: Reproductive Sciences

Article Title: CHAF1A Promotes Preadipocyte Differentiation and Contributes to Macrosomia in Gestational Diabetes Mellitus

doi: 10.1007/s43032-025-01946-z

Figure Lengend Snippet: Effects of CHAF1A overexpression on key factors involved in preadipocyte differentiation. A PPAR-γ, SREBP1, FASN, CEBPα, and LPL mRNA; B Western blotting. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The primary antibodies included: CHAF1A (1:1000), PPAR-γ (16,643–1-AP, 1:1000, Proteintech), SREBP1 (14,088–1-AP, 1:1000, Proteintech), FASN (10,624–2-AP, 1:1000, Proteintech), CEBPα(29,388–1-AP, 1:1000, Proteintech), LPL (28,602–1-AP, 1:1000, Proteintech) and β-Actin (66,009–1-Ig, 1:1000, Proteintech) as a loading control.

Techniques: Over Expression, Western Blot

Effects of CHAF1A knockdown on preadipocyte proliferation and differentiation. A Cell morphology under fluorescence microscope; B CHAF1A mRNA; C Western blotting; D CCK8 assays; E Glycerin triglyceride content assays; F Oil Red O staining. *** P < 0.001, ** P < 0.01, * P < 0.05

Journal: Reproductive Sciences

Article Title: CHAF1A Promotes Preadipocyte Differentiation and Contributes to Macrosomia in Gestational Diabetes Mellitus

doi: 10.1007/s43032-025-01946-z

Figure Lengend Snippet: Effects of CHAF1A knockdown on preadipocyte proliferation and differentiation. A Cell morphology under fluorescence microscope; B CHAF1A mRNA; C Western blotting; D CCK8 assays; E Glycerin triglyceride content assays; F Oil Red O staining. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The primary antibodies included: CHAF1A (1:1000), PPAR-γ (16,643–1-AP, 1:1000, Proteintech), SREBP1 (14,088–1-AP, 1:1000, Proteintech), FASN (10,624–2-AP, 1:1000, Proteintech), CEBPα(29,388–1-AP, 1:1000, Proteintech), LPL (28,602–1-AP, 1:1000, Proteintech) and β-Actin (66,009–1-Ig, 1:1000, Proteintech) as a loading control.

Techniques: Knockdown, Fluorescence, Microscopy, Western Blot, Staining

Effects of CHAF1A knockdown on key factors involved in preadipocyte differentiation. A PPAR-γ, SREBP1, FASN, CEBPα, and LPL mRNA; B Western blotting. *** P < 0.001, ** P < 0.01, * P < 0.05

Journal: Reproductive Sciences

Article Title: CHAF1A Promotes Preadipocyte Differentiation and Contributes to Macrosomia in Gestational Diabetes Mellitus

doi: 10.1007/s43032-025-01946-z

Figure Lengend Snippet: Effects of CHAF1A knockdown on key factors involved in preadipocyte differentiation. A PPAR-γ, SREBP1, FASN, CEBPα, and LPL mRNA; B Western blotting. *** P < 0.001, ** P < 0.01, * P < 0.05

Article Snippet: The primary antibodies included: CHAF1A (1:1000), PPAR-γ (16,643–1-AP, 1:1000, Proteintech), SREBP1 (14,088–1-AP, 1:1000, Proteintech), FASN (10,624–2-AP, 1:1000, Proteintech), CEBPα(29,388–1-AP, 1:1000, Proteintech), LPL (28,602–1-AP, 1:1000, Proteintech) and β-Actin (66,009–1-Ig, 1:1000, Proteintech) as a loading control.

Techniques: Knockdown, Western Blot

Analysis of transcriptome sequencing results after CHAF1A knockdown. A Intersection analysis of differential genes; B Annotation of top 30 pathways possibly involved by GO analysis; C Annotation of the top 30 pathways possibly involved by KEGG analysis; D Western blotting of related pathways. *** P < 0.001, * P < 0.05

Journal: Reproductive Sciences

Article Title: CHAF1A Promotes Preadipocyte Differentiation and Contributes to Macrosomia in Gestational Diabetes Mellitus

doi: 10.1007/s43032-025-01946-z

Figure Lengend Snippet: Analysis of transcriptome sequencing results after CHAF1A knockdown. A Intersection analysis of differential genes; B Annotation of top 30 pathways possibly involved by GO analysis; C Annotation of the top 30 pathways possibly involved by KEGG analysis; D Western blotting of related pathways. *** P < 0.001, * P < 0.05

Article Snippet: The primary antibodies included: CHAF1A (1:1000), PPAR-γ (16,643–1-AP, 1:1000, Proteintech), SREBP1 (14,088–1-AP, 1:1000, Proteintech), FASN (10,624–2-AP, 1:1000, Proteintech), CEBPα(29,388–1-AP, 1:1000, Proteintech), LPL (28,602–1-AP, 1:1000, Proteintech) and β-Actin (66,009–1-Ig, 1:1000, Proteintech) as a loading control.

Techniques: Sequencing, Knockdown, Western Blot